The objective of this project is to develop biosensors based on lateral flow immunoassays targeting signaling molecules. These compounds are released at early stages of quorum sensing. The strips will be coupled to an electromagnetic sensor based on impedance measurements. Sensitivity requirements will be explored by synergic effects among nanoparticles and transducers. The sensing platforms will be used to test novel biocides and evaluate antimicrobial susceptibility.
My name is Shayesteh Bazsefidpar. I am from Iran. I have completed my master degree in Chemical Engineering (biotechnology) from Chemistry and Chemical Engineering research center of Iran (CCERCI). I was a member of Biosurfactant Team at CCERCI with the aim of investigation, characterization and production of biosurfactants from bacterial strains, and my project was a part of this research project. My master dissertation focused on production and optimization of rhamnolipid produced by P. aeruginosa in a lab-scale fermenter. I also had collaboration in this research group on the other project entitled: “Investigation of antimicrobial and anti-adhesive effect of rhamnolipid produced by Pseudomonas aeruginosa on biofilm formation in food packaging”. I am very glad to be one of the researchers in the European network “Break Biofilms”, in order to the detection of bacterial biofilms at early stage of formation using biosensors.
This project aims to develop effective formulations based on nanoparticles and nanovesicles for breaking biofilms and/or prevent their growth. Properties of surfaces modified by antibacterial nanovesicles and/ or conjugated with antimicrobial inorganic nanoparticles will be studied. The surface of the nanomaterials will be characterized by XPS. The size, composition, and charge of the vesicle, pH and temperature sensitivity, fluidity and permeability of the membrane of nanocapsules will be tuned for a range of selected applications.
I completed my master degree in Pharmaceutical Chemistry and Technology at the University of Bari, Italy, in 2019.
During my master internship I worked on antimicrobial nanomaterials, the thesis topic was: “Electrodecoration and characterization of iron oxide nanoparticles with bioactive nanophases for targeted antimicrobial materials”. I synthesized bioactive copper nanoparticles and electrodecorated them with iron oxide to obtain new nanomaterials with improved antimicrobial properties. Thanks to the encouraging results I attended oral presentations in workshops.
My pharmaceutical background led me to continue to study new approaches to eradicate biofilms and I am glad to be involved in the European network “Breaking Bad Biofilms”. The aim of my project is to develop new formulations of antibiotic nanovesicles to kill or prevent biofilms.
Innovative electrochemical probe microscopes and nanoscale electrochemical techniques will be used to understand microorganism adhesion to surfaces and their activity at different stages of biofilm formation. In particular, multi-functional electrochemical imaging probes (‘Lab-on-a-Tip’) will be used to map and measure chemical fluxes associated with bacterial function at the nanoscale and to deliver antibacterial agents locally and precisely. This project provides the ESR with an exciting opportunity to become expert in frontier nanoscale electrochemical and surface chemistry methods of wide applicability from the life sciences to materials science and electrochemistry.
My name is Gözde. I am from Istanbul, Turkey. I completed my undergraduate study in Chemical Engineering in Istanbul University in 2016. After, I received a scholarship from Education, Audiovisual and Culture Executive Agency of European Comission in Erasmus Mundus Joint Master Degree in Nanomedicine for Drug Delivery. During my Master degree, I studied in France, Italy and Greece. I did poster and oral presentations in workshops during the Master programme and I worked on “liposomal antibiotic delivery system towards Gram-negative bacteria” during my Master thesis. I am very keen to search about antimicrobial resistance and developing technologies to eradicate and understand antimicrobial resistance. In my free time I swim, travel and visit art museums.
The aim of this project is the development of on-line electrochemical sensors which target biofilms commonly found in potable water supplies. An innovative biofilm sensing technology will be designed and fabricated; the factors governing the relationship between sensor response, biofilm growth and electrochemical activity will be determined. In addition, conducting diamond electrode technology will be implemented as a means to both destroy biofilms and clean the sensors in-situ. This procedure will optimize electrolysis of aqueous electrolyte to produce ozone and hydroxyl radicals.
My name is Irina and I hail from sunny, beautiful Dominican Republic. I received a BS in Chemistry from Utah State University, where I worked in prof. Yujie Sun’s lab synthesizing electrocatalysts for water splitting and biomass upgrading. I went on to obtain an MS in Chemistry with a concentration in Biochemistry. For my MS project, I worked with prof. Glen O’Neil at Montclair State University developing and characterizing a light-addressable electrode for neurotransmitter sensing. I am thrilled about the opportunity of learning more about electrochemical sensing, diamond electrodes and cell biology while contributing to Break Biofilms. In my spare time, I enjoy drawing, reading and learning about other cultures.
The aim of this project is using advanced hybrid analytical methods such as combined scanning probe techniques to study bacterial cell adhesion. Single cell force spectroscopy using novel colloidal AFM-SECM probes with potentiometric AFM-SECM probes for localized pH measurements will be further developed and applied to biolfilm studies. Nanoparticles release kinetics and on surface morphology changes in biofilms will be characterized. Stimulation experiments to locally change the microenvironment of the biofilm or bacterial aggregates or by releasing silver ions AFM tip-integrated electrode will be implemented. Changes induced may then be monitored using a multifunctional platform via IR-ATR.
Hello, my name is Giada. I completed my master in Chemistry at Universidad de La Laguna (Spain) in 2017 and the thesis topic was the synthesis and characterization of carbohydrate-based triazoles and the study of their gelling properties. With this work a publication was achieved. After completing the master I trained in Scanning Electrochemical Microscopy (SECM) applied in corrosion processes in the Group of Electrochemistry at Universidad de La Laguna. My thesis is focused on the study of bacterial cell adhesion processes using hybrid AFM-SECM techniques; this challenging project combines my two passions: biochemistry and micro-electrochemistry and I am very glad to be part of it. I hope to provide a small piece to contribute to the knowledge of this intriguing microscopic world.
The main objective of this project is the development of advanced mid-infrared spectroscopic techniques for studying biofilm formation in combination with orthogonal sensing concepts such as AFM, luminescence, and electrochemical analysis techniques. Next to investigating biofilm formation, antimicrobial films and their release characteristics will be studied, and complemented by multivariate data evaluation strategies and data classification algorithms enabling advancing multi-parametric data mining.
I completed my undergraduate studies in the field of Engineering Chemistry and I graduated in Master in the field of Analytical and Environmental Chemistry at the University of Prishtina “Hasan Prishtina”, Kosovo, in 2017. During my Master, I was a beneficiary of a CEEPUS scholarship, attending the fourth semester of Master studies at “Karl Franzens” University of Graz, Austria where I did my Master thesis work under supervision of Prof. Dr. Anton Huber. I gained hands-on experiences on various analytical sepacation techniques (SEC, AF4, viscosity & rheology of (semi) dense systems upon oscillating stress, etc) for the development of polymer and hydrocolloidal characteristics of a young-fermenter Xanthan polysaccharide. As a result of a sucessful research done, I am a co-author of a book chapter and due to my experience, I have publication on the evaluation of antibacterial activity of organic plant extracts. I am really excited to share and continue exploring in the field of analytical chemistry platform based on Infrared Attenuated Total Reflection Spectroscopy and combination techniques for studying the chemical and physical properties of biofilms. Taking of chance to be a part of The Breaking Biofilms Project is one of the most satisfactory opportunity of my research career
Several classes of nanoantimicrobial thin films (NAMs) will be developed, characterized (XPS, IR, UV-Vis, TEM, SEM, AFM, etc.) and applied to the protection of surfaces. Physical, wet chemical, electrochemical and hybrid methods will be used for the synthesis/deposition of NAMs, and the relevant outcomes will be critically compared in terms of (i) material’s stability, (ii) ion release properties, (iii) absence of whole-particle release, etc. NAMs composed of Ag or Cu, or ZnO nanoparticles dispersed in organic or inorganic matrices will be obtained and tested.
Hi! I am Syed Imdadul Hossain. I completed Bachelor degree in Chemical Engineering at JUST, Bangladesh. I graduated Master’s degree in Enery Science and Engineering at DGIST, South Korea. During my Master’s study, I worked on fabrication of alternative composite (incorporation of inorganic nanomaterials) membrane for either redox flow battery or fuel cell. And successfully published 4 (2 as author and another 2 as co-author) research paper in prestigious journal. During that period, I was awarded Korean National Scholarship, DGIST excellent research award 2018, and so on. After that, I moved to MLU, Germany and worked as a Graduate research fellow. I have experience running FTIR, XRD, UV, and TGA.
Currently, I am working on “Nanomaterials Films: Deposition, Characterization And Evaluation of Their Bio Activity Properties, Including Ion And Nanoparticle Release” at UNIBA Italy, within BBB research project.
Development of novel synergistic nanoantimicrobials. Different classes of organic or inorganic bioactive nanoparticles will be prepared, characterized, and tested on fighting antimicrobial/antibiotic resistance. Several bioactive dispersing matrixes/polymers will be used to embed and stabilize the nanoparticles. Synergistic combination of 2 or more bioactive components within the same nanoantimicrobials will be investigated. Conventional disinfection agents such as biopolymers and quaternary ammonium salts will be tested as nanoantimicrobials coadjuvant. Composite nanoantimicrobials will be characterized by spectroscopic, morphological, and microbiological techniques at different stages of their storage and use.
In 2016 I obtained a Bachelor degree in Chemical Technology in State Pharmaceutical University, St. Petersburg, Russia. The following year after obtaining a BSc was dedicated to work in biotechnological company Biocad, Russia, as an engineer of Active Pharmaceutical Ingridients (API) for cancer treatment. In 2017 I was awarded with Paris-Saclay scholarship to pursue a Master studies in France and Italy within SERP+ Erasmus Dual Master degree program in Physical Chemistry (University Paris-Sud) and Material Sciences (University of Genoa). My Master thesis was carried on the Institute of Molecular Sciences in Orsay, France, and dedicated to chemical synthesis and testing of biocompatible antimicrobial agents. Within BBB research project in UniBa I am working on the development and full characterization of novel nanoantimicrobials (NAMs) which consist of 2 or/and more active components.
In my spare time I love to listen to live music, read good literature and have nice talks with interesting people. My sources of inspiration and motivation are my family, friends, colleagues and nature.
The aim of this project is the development of specific in-vivo electrochemical assays for immediate analysis of bacterial growth on surfaces, the quality of bacterial films, and biofilm degradation by biocides by targeting the intracellular signaling pathways and their response to environmental changes. The ESR will develop innovative technologies based on electronic (E) beacons for intracellular monitoring of the bacterial metabolism. E-beacons will rely on redox-labelled dendrimers and DNA and peptide wires tethered to electrodes and be used as nano-electrodes transfected inside the bacterial cells.
Hi! I am Rimsha stemming from Karachi, Pakistan. I completed my undergrad in Biomedical Engineering from NEDUET, 2016. Right after my Bachelors I was appointed as a Biomedical Engineer in a start-up, Tech4Life dealing mainly with biosensors. During this short period of employment, I realized the eminent gap between industry and research, and decided to pursue Masters in emerging technologies. In the following year of 2017, I got accepted in Erasmus Mundus Masters of Nanoscience and Nanotechnology; a joint degree program with KU Leuven as the host university. For the specialization of Bio-nanotechnology, I opted University Grenoble Alps, France, where I conducted my master thesis as well. The project was a collaboration between the labs of IMEP-LAHC and G2E, specialized in the fields of photonics, micro, nano-fabrication and electronics. My thesis was dedicated to the design and fabrication of a cost effective and simple sensor utilizing impedance spectroscopy as a detection mechanism for bacterial contamination in processed food. Building up on the interest of working with biosensors, in the Break Biofilms consortium I am working with nano-bio electrodes for intracellular analysis of biofilm development and degradation at Aarhus University, Denmark. In my free time I love to paint and do perspective photography.
This project aims to develop antimicrobial formulations, based on functional enzymes and reactive products of their biotransformation, and such encapsulated biocides as antibiotics and antimicrobial proteins/peptides, broadly applicable for breaking the biofilm and its growth on different surfaces: steel (anticorrosion); nanostructured conductive surfaces (coating of implants); ceramics and thin plastic layer surfaces (industrial and medical cleaning). The ESR will develop formulations that in combination with optimized immobilization and encapsulation technologies will produce antifouling surface effects against a wide range of microorganisms.
I come from a small and sunny coastal city situated at the southern tip of peninsula Istria in Croatia, called Pula. During my undergraduate studies, Zagreb, the capital, became my second home. There I founded my practical and theoretical knowledge in chemistry, especially inorganic chemistry and biochemistry. In September 2019, I completed my Masters in Chemistry at the University of Zagreb and carried out a thesis focused on the cocrystallization of donepezil and its chloride salt with perflourinated halogen bond donors. Throughout my studies, I have had simultaneous work experience at the R&D Physical Laboratory of pharmaceutical company PLIVA d.o.o., Zagreb, Croatia and have actively participated in Assoc. Prof. Dominik Cinčić’s research group at alma mater. On top of that, I had the opportunity to do an internship at the Central European Institute of Technology in Prof. Jozef Kaiser’s research group, situated in Brno, Czech Republic.
Above everything else, these experiences played an important factor in choosing my next career move, which led me to pursue a PhD position in this European Innovative Training Network (ITN). Consequently, since mid-February 2020, I have taken part in the BBB network at Aarhus University, Denmark. The main goal of my project is to introduce new methods for biofilm decomposition and prevention of their growth, based on antimicrobial properties of encapsulated enzymes and nanoparticles. As the biofilm formation represents a serious problem in modern society, I am looking forward to giving my contribution to this project and provide both, industry and scientific community, valuable results during my PhD.
The objective of this project is to create highly sensitive sensors to detect ultralow concentrations of pathogens and to understand their redox properties. The ESR will synthesize and characterise transition metal complex mediators, optimize the performance of redox mediators for electron transfer to/from microbial biofilms, and evaluate the utility of the optimised mediators for the high sensitivity, direct detection of biofilms through redox, and electrochemiluminescence detection. A particular focus will be on wireless or bipolar electrochemical detection.
I studied Biology at the University of the Basque Country (Bilbao, Spain) obtaining a specialization in Genetics, Cell and Molecular Biology. Due to the fact that I was interested in the applications of my knowledge, I enrolled the University of Oviedo in the Master in Biotechnology of Environment and Health, were I acquired a solid formation about biosensors. In 2017 I did my first Practicum of the Master’s degree at the Nanomaterials and Nanotechnology Research Centre (CINN-CSIC, Asturias) developed a strong interest in how to avoid biofilms formation and bacterial a growth using nanomaterials. Nevertheless, I got a different insight about bacterial growth when I did my Master’s Final Thesis (2019) at the Dairy Products Institute of Asturias (IPLA-CSIC, Asturias) in regards to virus induction in lactococcal strains. The stays at different laboratories have provided me with the experience and skills for research in the field of both microbiology and molecular biology but also knowledge about how to do good research, the positive aspects of working in a multicultural environment and the value of having a multidisciplinary background. Now, I’m lucky to say that I have the chance to combine both microbiology and biosensors in order to solve problems such as biofilm formation and provide to industry the best solutions under the Break Biofilms Training Network.
This project aims to develop novel point-of-use electrochemical and electrochemiluminescence based sample-to-answer devices to detect and quantify bacteria present within biofilms relevant to the biomedical, as well as, food and drink industries. The overall goal is to dramatically reduce the sample-to-answer time allowing contamination to be quickly identified. The fabrication of highly efficient capture surfaces using self-assembly as well as 2D and 3D printing will be a particular focus.
I completed my integrated Masters in Chemistry at University of Warwick in 2019. As part of my degree I completed a research placement in Monash University, Melbourne, where I worked on developing electrode surfaces for electrocatalysed carbon dioxide reduction with Dr. Jie Zhang’s group. More recently I completed my masters project under supervision of Prof. Julie Macpherson. The project was on developing electrochemical free chlorine sensors. My thesis focused on tracking the pH dependant speciation of Hypochlorite / Hypochlorous acid using boron doped diamond as the sensing material. I am excited at the prospect of applying electrochemistry to the detection of bacterial biofilms. In my free time I enjoy live music, travel, Irish dance and special effects make up.
The goal of this project is to develop a novel set of phage-based antimicrobial products to be used as disinfectants against S. aureus and S. epidermidis in clinical settings and food industries. The stability of bacteriophages on different surfaces (including food matrices) will be determined, and the putative interactions between phage-derived anti-biofilm compounds and antibiotics/disinfectants will be investigated. The cytotoxicity of antimicrobial strategies based on phages and/or nanoparticles will be tested on cell lines by using the RTCA system.
In 2018 I graduated in Biotechnology at the University of Minho, Braga. My Master Thesis project was named “Interaction of phages with biofilms of single and mixed multidrug resistant species involved in pneumonia infections”. During this work I was integrated in the BBiG Bacteriophage Biotechnology Group of Center of Biological Engineering.
Since October 2019, I am involved in a European network called Breaking Bad Biofilms and my project is “Development of novel bacteriophage-based products for biofilm removal” which work is being performed in Instituto de Productos Lácteos de Asturias (IPLA, CSIC). The main goal of this project is to develop a novel set of phage-based antimicrobial products to be used as disinfectants against S. aureus and S. epidermidis in clinical settings and food industries.
The goal of this project is to investigate the biofilm formation ability of lactic acid bacteria (LAB) strains able to produce biogenic amines (BA). The ability of these bacteria to form biofilm favours their presence as contaminant microorganisms in food industry equipment and medical devices. For this reason, it is necessary the development of specific strategies to eliminate these bacteria. The genome sequencing of BA-producing LAB will be performed, and the research on the regulation of genes related to surface adhesion and biofilm formation will be carried out.
Hi! I am Agustina, from Argentina. My country is in South America and is amazing for the variety of natural landscapes and its culture. I completed my degree in Nutrition at Universidad Nacional del Litoral (Santa Fe, Argentina) in 2017. My thesis topic was a Technological and in vitro functional characterization of novel bifidobacteria isolated from baby monkeys as potential probiotics in food. With this work a publication was achieved.
Since January 2020, I am involved in a European network called Breaking Bad Biofilms and my project is “Understanding lactic acid bacteria biofilm formation”, which is being performed in Instituto de Productos Lácteos de Asturias (IPLA, CSIC). The goal of this project is to know the mechanism and factors affecting biofilm formation in lactic acid bacteria.
I love what I do in the lab, but in my free time, I prefer acting, reading or riding a bike.
The main goal is the development of a non-destructive analysis tool to detect complex biofilms in different surfaces in contact with food, based on the use of different spectroscopy techniques (mainly NIR, hyperspectral) and the following chemometric analysis using advanced mathematical, statistical and computer science (Artificial intelligence) methodologies. The ESR will develop complex biofilms considering key parameters as maturation degree and surface material; following will design an analytical methodology to evaluate biofilms; then, correlation between generated biofilms and spectra will be established using chemometrics. Finally, new tool will be validated in ASINCAR’s pilot plant.
Hi! My name is Nazan and I come from Istanbul, Turkey. I obtained a BSc degree in Chemical Engineering from Ege University with one year of exchange period at the University of Porto. After finishing my undergraduate studies, I earned a scholarship from European Commission for a joint master’s degree in Chemical Innovation and Regulation. I took my courses at the University of Algarve and conducted my dissertation research at Alma Mater Studiorum – University of Bologna.
I participated in academia-industry collaborations actively and performed several industrial scale project writing and follow-up services, internships and externships in Istanbul, Lisbon and Bologna. During my previous scientific and technical activities, I focused on optimization, experimental design, quality by design, mathematical modelling, chemometrics and scale up studies applied mainly on several issues in pharmaceutical, coating and materials sciences and industries.
I had an opportunity to get a PhD position in ASINCAR – Asturian Agrifood Cluster by February 2020. As of starting it, I aim to pursue my interest in multivariate statistical analysis and data interpretation on near-infrared spectroscopy (NIRs) by bringing it into microbiology field. I am happy to utilize this project and network also for extending the scope of my professional experience into food sector.
In my spare time I enjoy travelling, drawing acrylic paints and hiking.
BREAKING BAD BIOFILMS.
INNOVATIVE ANALYSIS AND DESIGN RULES FOR
NEXT-GENERATION ANTIFOULING INTERFACES
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 813439.